PROM1 monoclonal antibody (M10), clone 3D4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PROM1 expression in transfected 293T cell line by PROM1 monoclonal antibody (M10), clone 3D4.
Lane 1: PROM1 transfected lysate (Predicted MW: 96.3 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence (Circulating Cancer Stem Cell)
Representative images of circulating lung cancer cells (white arrow) from patient with lung cancer. Circulating lung cancer cells were detected by using immunofluorescence staining for CSV (FITC, green), CD45 (PE, orange),CD133 (APC,red) and Nucleus (Hoechst 33342, blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to PROM1 on MCF-7 cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PROM1.
Immunogen
PROM1 (NP_006008.1, 747 a.a. ~ 760 a.a) partial recombinant protein.
Sequence
IGYFEHYLQWIEFSC
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PROM1 expression in transfected 293T cell line by PROM1 monoclonal antibody (M10), clone 3D4.
Lane 1: PROM1 transfected lysate (Predicted MW: 96.3 KDa).
Lane 2: Non-transfected lysate.
ELISA
Immunofluorescence (Circulating Cancer Stem Cell)
Representative images of circulating lung cancer cells (white arrow) from patient with lung cancer. Circulating lung cancer cells were detected by using immunofluorescence staining for CSV (FITC, green), CD45 (PE, orange),CD133 (APC,red) and Nucleus (Hoechst 33342, blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to PROM1 on MCF-7 cell . [antibody concentration 10 ug/ml] -
Gene Info — PROM1
Entrez GeneID
8842GeneBank Accession#
NM_006017.2Protein Accession#
NP_006008.1Gene Name
PROM1
Gene Alias
AC133, CD133, MSTP061, PROML1, RP41
Gene Description
prominin 1
Omim ID
604365Gene Ontology
HyperlinkGene Summary
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
hProminin|hematopoietic stem cell antigen|prominin-like 1
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Interactome
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Disease
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