WISP3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WISP3 full-length
Host
Human
Theoretical MW (kDa)
41.4
Interspecies Antigen Sequence
Mouse (78); Rat (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WISP3 antibody (H00008838-B01) by Western Blots.
SDS-PAGE Gel
WISP3 transfected lysate.
Western Blot
Lane 1: WISP3 transfected lysate ( 41.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WISP3
Entrez GeneID
8838GeneBank Accession#
NM_198239.1Protein Accession#
NP_937882.1Gene Name
WISP3
Gene Alias
CCN6, LIBC, MGC125987, MGC125988, MGC125989, PPAC, PPD
Gene Description
WNT1 inducible signaling pathway protein 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017037|OTTHUMP00000017038|OTTHUMP00000040421
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