CFLAR monoclonal antibody (M01), clone 2F12
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CFLAR.
Immunogen
CFLAR (AAH01602, 178 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AGTSYRNVLQAAIQKSLKDPSNNFRLHNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYKMKSKPLGICLIIDCIGNETELLRDTFTSLGYE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (67); Rat (68)
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CFLAR monoclonal antibody (M01), clone 2F12. Western Blot analysis of CFLAR expression in K-562.Western Blot (Cell lysate)
CFLAR monoclonal antibody (M01), clone 2F12 Western Blot analysis of CFLAR expression in IMR-32 ( Cat # L008V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CFLAR expression in transfected 293T cell line by CFLAR monoclonal antibody (M01), clone 2F12.
Lane 1: CFLAR transfected lysate (Predicted MW: 55.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CFLAR on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CFLAR is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CFLAR
Entrez GeneID
8837GeneBank Accession#
BC001602Protein Accession#
AAH01602Gene Name
CFLAR
Gene Alias
CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS
Gene Description
CASP8 and FADD-like apoptosis regulator
Omim ID
603599Gene Ontology
HyperlinkOther Designations
FADD-like anti-apoptotic molecule|caspase-related inducer of apoptosis|inhibitor of FLICE|usurpin beta
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Interactome
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Pathway
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Disease
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