SAP30 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SAP30 transfected lysate.
Western Blot
Lane 1: SAP30 transfected lysate ( 23.30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SAP30 full-length
Host
Human
Theoretical MW (kDa)
23.3
Interspecies Antigen Sequence
Mouse (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SAP30 antibody (H00008819-D01P) by Western Blots.
SDS-PAGE Gel
SAP30 transfected lysate.
Western Blot
Lane 1: SAP30 transfected lysate ( 23.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SAP30
Entrez GeneID
8819GeneBank Accession#
NM_003864.3Protein Accession#
NP_003855.1Gene Name
SAP30
Gene Alias
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Gene Description
Sin3A-associated protein, 30kDa
Omim ID
603378Gene Ontology
HyperlinkGene Summary
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq
Other Designations
Sin3 corepressor complex subunit SAP30|Sin3-associated polypeptide, 30kDa|histone deacetylase complex subunit SAP30
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