CCNK monoclonal antibody (M01), clone 3B7-1B9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CCNK.
Immunogen
CCNK (AAH15935, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPLILLQGWACRQPATHLLPSPLEDSLLCPRPFPHPACLQLGGWGGQPG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (64.68 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CCNK monoclonal antibody (M01), clone 3B7-1B9. Western Blot analysis of CCNK expression in PRC/PRF/5.Western Blot (Transfected lysate)
Western Blot analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M01), clone 3B7-1B9.
Lane 1: CCNK transfected lysate(41 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CCNK is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CCNK over-expressed 293 cell line, cotransfected with CCNK Validated Chimera RNAi ( Cat # H00008812-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CCNK monoclonal antibody (M01), clone 3B7-1B9 (Cat # H00008812-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CCNK
Entrez GeneID
8812GeneBank Accession#
BC015935Protein Accession#
AAH15935Gene Name
CCNK
Gene Alias
CPR4, MGC9113
Gene Description
cyclin K
Omim ID
603544Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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The human HECA interacts with cyclins and CDKs to antagonize Wnt-mediated proliferation and chemoresistance of head and neck cancer cells.
Dowejko A, Bauer R, Bauer K, Muller-Richter UD, Reichert TE.
Experimental Cell Research 2012 Mar; 318(5):489.
Application:IP, Human, PCI13, PCI9 cells.
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The human HECA interacts with cyclins and CDKs to antagonize Wnt-mediated proliferation and chemoresistance of head and neck cancer cells.
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