FPGT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
FPGT transfected lysate.
Western Blot
Lane 1: FPGT transfected lysate ( 65.45 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FPGT full-length
Host
Human
Theoretical MW (kDa)
65.45
Interspecies Antigen Sequence
Mouse (75); Rat (72)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FPGT antibody (H00008790-B01) by Western Blots.
SDS-PAGE Gel
FPGT transfected lysate.
Western Blot
Lane 1: FPGT transfected lysate ( 65.45 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FPGT
Entrez GeneID
8790GeneBank Accession#
NM_003838.2Protein Accession#
NP_003829.2Gene Name
FPGT
Gene Alias
GFPP
Gene Description
fucose-1-phosphate guanylyltransferase
Omim ID
603609Gene Ontology
HyperlinkGene Summary
L-fucose is a key sugar in glycoproteins and other complex carbohydrates since it may be involved in many of the functional roles of these macromolecules, such as in cell-cell recognition. The fucosyl donor for these fucosylated oligosaccharides is GDP-beta-L-fucose. There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta-L-fucose. The protein encoded by this gene participates in an alternate pathway that is present in certain mammalian tissues, such as liver and kidney, and appears to function as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate, and then condensation of the beta-L-fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. [provided by RefSeq
Other Designations
GDP-beta-L-fucose pyrophosphorylase|OTTHUMP00000011173|fucose-1-phosphate guanyltransferase
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Interactome
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Pathway
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