RIPK2 monoclonal antibody (M05), clone 7F5
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RIPK2.
Immunogen
RIPK2 (AAH04553, 431 a.a. ~ 540 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (85)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RIPK2 monoclonal antibody (M05), clone 7F5 Western Blot analysis of RIPK2 expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — RIPK2
Entrez GeneID
8767GeneBank Accession#
BC004553Protein Accession#
AAH04553Gene Name
RIPK2
Gene Alias
CARD3, CARDIAK, CCK, GIG30, RICK, RIP2
Gene Description
receptor-interacting serine-threonine kinase 2
Omim ID
603455Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq
Other Designations
CARD-carrying kinase|CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase|growth-inhibiting gene 30|receptor interacting protein 2|receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP
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Interactome
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Pathway
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Disease
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Publication Reference
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A proteomic analysis of C-reactive protein stimulated THP-1 monocytes.
Eisenhardt SU, Habersberger J, Oliva K, Lancaster GI, Ayhan M, Woollard KJ, Bannasch H, Rice GE, Peter K.
Proteome Science 2011 Jan; 9(1):1.
Application:WB, Human, THP-1 cells.
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A proteomic analysis of C-reactive protein stimulated THP-1 monocytes.
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