ADAM15 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ADAM15 full-length
Host
Human
Theoretical MW (kDa)
85.03
Interspecies Antigen Sequence
Mouse (75); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ADAM15 antibody (H00008751-B01) by Western Blots.
SDS-PAGE Gel
ADAM15 transfected lysate.
Western Blot
Lane 1: ADAM15 transfected lysate ( 85.03 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ADAM15
Entrez GeneID
8751GeneBank Accession#
NM_207191.1Protein Accession#
NP_997074.1Gene Name
ADAM15
Gene Alias
MDC15
Gene Description
ADAM metallopeptidase domain 15
Omim ID
605548Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000015780|a disintegrin and metalloproteinase domain 15|a disintegrin and metalloproteinase domain 15 (metargidin)|metalloprotease RGD disintegrin protein|metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15|metargidin
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Interactome
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Disease
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