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Last updated: 2017/5/28

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CTSF 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00008722-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CTSF full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 53.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CTSF antibody (H00008722-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00008722-T01
    CTSF transfected lysate.
    Western Blot
    QC Testing of H00008722-T01
    Lane 1: CTSF transfected lysate ( 53.4 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 8722
  • Gene Name:
  • CTSF
  • Gene Alias:
  • CATSF
  • Gene Description:
  • cathepsin F
  • Gene Summary:
  • Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq
  • Other Designations:
  • -
  • Gene Pathway
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