EDF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
EDF1 transfected lysate
Western Blot
Lane 1: EDF1 transfected lysate ( 16 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EDF1 full-length
Host
Human
Theoretical MW (kDa)
16
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EDF1 antibody (H00008721-B02) by Western Blots.
SDS-PAGE Gel
EDF1 transfected lysate
Western Blot
Lane 1: EDF1 transfected lysate ( 16 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EDF1
Entrez GeneID
8721GeneBank Accession#
NM_003792.2Protein Accession#
NP_003783.1Gene Name
EDF1
Gene Alias
EDF-1, MBF1, MGC9058
Gene Description
endothelial differentiation-related factor 1
Omim ID
605107Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000022616|OTTHUMP00000022617|multiprotein bridging factor 1|multiprotein bridging factor-1
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