B3GALNT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-B3GALNT1 full-length
Host
Human
Theoretical MW (kDa)
39.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-B3GALNT1 antibody (H00008706-B01) by Western Blots.
SDS-PAGE Gel
B3GALT3 transfected lysate.
Western Blot
Lane 1: B3GALT3 transfected lysate ( 39.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — B3GALNT1
Entrez GeneID
8706GeneBank Accession#
BC028571.1Protein Accession#
-Gene Name
B3GALNT1
Gene Alias
B3GALT3, GLCT3, GLOB, Gb4Cer, P, P1, beta3Gal-T3, galT3
Gene Description
beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all. Multiple transcript variants that are alternatively spliced in the 5' UTR have been described; they all encode the same protein. [provided by RefSeq
Other Designations
P antigen synthase|P blood group globoside|UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 1|UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 3 (Globoside blood group)|UDP-GalNAc:betaGlcNAc beta-1,3-galactosaminyltransferase, polypeptide 1
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Interactome
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Pathway
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