B3GALT4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-B3GALT4 full-length
Host
Human
Theoretical MW (kDa)
41.5
Interspecies Antigen Sequence
Mouse (79); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-B3GALT4 antibody (H00008705-B02) by Western Blots.
SDS-PAGE Gel
B3GALT4 transfected lysate.
Western Blot
Lane 1: B3GALT4 transfected lysate ( 41.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — B3GALT4
Entrez GeneID
8705GeneBank Accession#
NM_003782Protein Accession#
NP_003773Gene Name
B3GALT4
Gene Alias
DJ1033B10.3, Gal-T2, GalT4, beta3GALT4, beta3Gal-T4
Gene Description
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
Omim ID
603095Gene Ontology
HyperlinkGene Summary
This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq
Other Designations
OTTHUMP00000029128|UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 4|beta-3-galactosyltransferase 4
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Interactome
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Disease
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