B4GALT3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-B4GALT3 full-length
Host
Human
Theoretical MW (kDa)
43.34
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-B4GALT3 antibody (H00008703-B01) by Western Blots.
SDS-PAGE Gel
B4GALT3 transfected lysate.
Western Blot
Lane 1: B4GALT3 transfected lysate ( 43.34 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — B4GALT3
Entrez GeneID
8703GeneBank Accession#
NM_003779.2Protein Accession#
NP_003770.1Gene Name
B4GALT3
Gene Alias
beta4Gal-T3
Gene Description
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
Omim ID
604014Gene Ontology
HyperlinkGene Summary
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. [provided by RefSeq
Other Designations
OTTHUMP00000032247|OTTHUMP00000032248|UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 3|UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3|beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 3
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Interactome
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Pathway
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