CDC23 monoclonal antibody (M08), clone 1F3
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More Files
- More Functions
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CDC23 is 3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CDC23 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.85 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CDC23.
Immunogen
CDC23 (NP_004652.1, 491 a.a. ~ 591 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
IYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.85 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CDC23 is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CDC23 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CDC23
Entrez GeneID
8697GeneBank Accession#
NM_004661Protein Accession#
NP_004652.1Gene Name
CDC23
Gene Alias
APC8
Gene Description
cell division cycle 23 homolog (S. cerevisiae)
Omim ID
603462Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq
Other Designations
anaphase-promoting complex subunit 8|cell division cycle protein 23
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Interactome
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Pathway
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