GALNT4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GALNT4 transfected lysate.
Western Blot
Lane 1: GALNT4 transfected lysate ( 66.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GALNT4 full-length
Host
Human
Theoretical MW (kDa)
66.6
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GALNT4 antibody (H00008693-B01) by Western Blots.
SDS-PAGE Gel
GALNT4 transfected lysate.
Western Blot
Lane 1: GALNT4 transfected lysate ( 66.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GALNT4
Entrez GeneID
8693GeneBank Accession#
BC036390.2Protein Accession#
AAH36390.1Gene Name
GALNT4
Gene Alias
GALNAC-T4, GalNAcT4
Gene Description
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)
Omim ID
603565Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. [provided by RefSeq
Other Designations
GalNAc transferase 4|UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase 4|polypeptide N-acetylgalactosaminyltransferase 4|protein-UDP acetylgalactosaminyltransferase 4
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Interactome
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Pathway
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Disease
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