VAMP8 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VAMP8 full-length
Host
Human
Theoretical MW (kDa)
11.11
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VAMP8 antibody (H00008673-B01) by Western Blots.
Western Blot
Lane 1: VAMP8 transfected lysate ( 11 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
VAMP8 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VAMP8
Entrez GeneID
8673GeneBank Accession#
BC001634Protein Accession#
AAH01634Gene Name
VAMP8
Gene Alias
EDB
Gene Description
vesicle-associated membrane protein 8 (endobrevin)
Omim ID
603177Gene Ontology
HyperlinkGene Summary
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex. [provided by RefSeq
Other Designations
endobrevin|vesicle-associated membrane protein 8
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Interactome
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