EIF4G3 monoclonal antibody (M03), clone 1H5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant EIF4G3.
Immunogen
EIF4G3 (AAH30578, 1 a.a. ~ 515 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNSQPQTRSPFAAGPRPPHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIMVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIISGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (76); Rat (71)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (82.39 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
EIF4G3 monoclonal antibody (M03), clone 1H5 Western Blot analysis of EIF4G3 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Cell lysate)
EIF4G3 monoclonal antibody (M03), clone 1H5. Western Blot analysis of EIF4G3 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of EIF4G3 expression in transfected 293T cell line by EIF4G3 monoclonal antibody (M03), clone 1H5.
Lane 1: EIF4G3 transfected lysate(55.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EIF4G3 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EIF4G3 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of EIF4G3 over-expressed 293 cell line, cotransfected with EIF4G3 Validated Chimera RNAi ( Cat # H00008672-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF4G3 monoclonal antibody (M03), clone 1H5 (Cat # H00008672-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — EIF4G3
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Interactome
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Disease
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