EIF3S1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EIF3S1 full-length
Host
Human
Theoretical MW (kDa)
28.49
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EIF3S1 antibody (H00008669-B01) by Western Blots.
SDS-PAGE Gel
EIF3J transfected lysate.
Western Blot
Lane 1: EIF3J transfected lysate ( 28.49 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EIF3J
Entrez GeneID
8669GeneBank Accession#
NM_003758.2Protein Accession#
NP_003749.2Gene Name
EIF3J
Gene Alias
EIF3S1, eIF3-alpha, eIF3-p35
Gene Description
eukaryotic translation initiation factor 3, subunit J
Omim ID
603910Gene Ontology
HyperlinkGene Summary
Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004 [PubMed 14688252]; Fraser et al., 2007 [PubMed 17588516]).[supplied by OMIM
Other Designations
eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)|eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa
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