DYNLL1 monoclonal antibody (M02), clone 1H7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of DYNLL1 expression in transfected 293T cell line by DYNLL1 monoclonal antibody (M02), clone 1H7.
Lane 1: DYNLL1 transfected lysate(10.4 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DYNLL1 is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to DYNLL1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (33.66 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DYNLL1.
Immunogen
DYNLL1 (NP_003737.1, 1 a.a. ~ 72 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKH
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DYNLL1 expression in transfected 293T cell line by DYNLL1 monoclonal antibody (M02), clone 1H7.
Lane 1: DYNLL1 transfected lysate(10.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DYNLL1 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DYNLL1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — DYNLL1
Entrez GeneID
8655GeneBank Accession#
NM_003746Protein Accession#
NP_003737.1Gene Name
DYNLL1
Gene Alias
DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, MGC126137, MGC126138, PIN, hdlc1
Gene Description
dynein, light chain, LC8-type 1
Omim ID
601562Gene Ontology
HyperlinkGene Summary
Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq
Other Designations
8 kDa dynein light chain|cytoplasmic dynein light polypeptide|dynein light chain 1|dynein, cytoplasmic, light polypeptide 1|protein inhibitor of neuronal nitric oxide synthase
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Interactome
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Disease
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