DDX3Y 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX3Y full-length
Host
Human
Theoretical MW (kDa)
72.71
Interspecies Antigen Sequence
Mouse (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX3Y antibody (H00008653-B01) by Western Blots.
SDS-PAGE Gel
DDX3Y transfected lysate.
Western Blot
Lane 1: DDX3Y transfected lysate ( 72.71 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX3Y
Entrez GeneID
8653GeneBank Accession#
NM_004660.2Protein Accession#
NP_004651.2Gene Name
DDX3Y
Gene Alias
DBY
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked
Omim ID
400010Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, Y chromosome|OTTHUMP00000034504
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