NUMB 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NUMB transfected lysate
Western Blot
Lane 1: NUMB transfected lysate ( 14.96 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NUMB full-length
Host
Human
Theoretical MW (kDa)
14.96
Interspecies Antigen Sequence
Mouse (90); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NUMB antibody (H00008650-B01) by Western Blots.
SDS-PAGE Gel
NUMB transfected lysate
Western Blot
Lane 1: NUMB transfected lysate ( 14.96 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NUMB
Entrez GeneID
8650GeneBank Accession#
BC033824Protein Accession#
AAH33824Gene Name
NUMB
Gene Alias
S171
Gene Description
numb homolog (Drosophila)
Omim ID
603728Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
numb homolog
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Interactome
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Pathway
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Disease
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