PPAP2A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPAP2A full-length
Host
Human
Theoretical MW (kDa)
32.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPAP2A antibody (H00008611-B01) by Western Blots.
SDS-PAGE Gel
PPAP2A transfected lysate.
Western Blot
Lane 1: PPAP2A transfected lysate ( 32.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPAP2A
Entrez GeneID
8611GeneBank Accession#
NM_003711Protein Accession#
NP_003702.2Gene Name
PPAP2A
Gene Alias
LLP1a, LPP1, PAP-2a, PAP2, PAP2a2, PAP2alpha2, PAPalpha1
Gene Description
phosphatidic acid phosphatase type 2A
Omim ID
607124Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of this gene is found to be regulated by androgen in a prostatic adenocarcinoma cell line. At least two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
lipid phosphate phosphohydrolase 1|lipid phosphate phosphohydrolase 1a|phosphatidic acid phosphatase 2a|phosphatidic acid phosphohydrolase type 2a|type 2 phosphatidic acid phosphohydrolase|type-2 phosphatidic acid phosphatase alpha
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Interactome
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Disease
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