RGS20 monoclonal antibody (M04), clone 3E10
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More Files
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Western Blot (Cell lysate)
RGS20 monoclonal antibody (M04), clone 3E10. Western Blot analysis of RGS20 expression in NIH/3T3 ( Cat # L018V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RGS20 is 0.3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to RGS20 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RGS20.
Immunogen
RGS20 (NP_003693, 78 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NQEDQRPTIASHELRADLPTWEESPAPTLEEVNAWAQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMACEELKKEANKNIIEEKARIIYEDYISILS
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RGS20 monoclonal antibody (M04), clone 3E10. Western Blot analysis of RGS20 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RGS20 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RGS20 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RGS20
Entrez GeneID
8601GeneBank Accession#
NM_003702Protein Accession#
NP_003693Gene Name
RGS20
Gene Alias
RGSZ1, ZGAP1
Gene Description
regulator of G-protein signaling 20
Omim ID
607193Gene Ontology
HyperlinkGene Summary
Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins are GTPase-activating proteins for Gi (see GNAI1; MIM 139310) and Gq (see GNAQ; MIM 600998) class G-alpha proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination.[supplied by OMIM
Other Designations
regulator of G-protein signalling 20|regulator of Gz-selective protein signaling
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Interactome
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Disease
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