PRPF18 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PRPF18 transfected lysate.
Western Blot
Lane 1: PRPF18 transfected lysate ( 39.9 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRPF18 full-length
Host
Human
Theoretical MW (kDa)
39.9
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRPF18 antibody (H00008559-B02) by Western Blots.
SDS-PAGE Gel
PRPF18 transfected lysate.
Western Blot
Lane 1: PRPF18 transfected lysate ( 39.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRPF18
Entrez GeneID
8559GeneBank Accession#
NM_003675Protein Accession#
NP_003666Gene Name
PRPF18
Gene Alias
FLJ10210, PRP18, hPrp18
Gene Description
PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae)
Omim ID
604993Gene Ontology
HyperlinkGene Summary
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq
Other Designations
OTTHUMP00000019146|PRP18 pre-mRNA processing factor 18 homolog|PRP18 pre-mRNA processing factor 18 homolog(PRPF18)
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Interactome
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Disease
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