CDC14A monoclonal antibody (M02), clone 1F11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of CDC14A expression in transfected 293T cell line by CDC14A monoclonal antibody (M02), clone 1F11.
Lane 1: CDC14A transfected lysate(66.6 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CDC14A on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CDC14A.
Immunogen
CDC14A (NP_003663, 431 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PFRLSSSLQGSAVTLKTSKMALSPSATAKRINRTSLSSGATVRSFSINSRLASSLGNLNAATDDPENKKTSSSSKAGFTASPFTNLLNGSSQPTTRNYPE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (71); Rat (70)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CDC14A expression in transfected 293T cell line by CDC14A monoclonal antibody (M02), clone 1F11.
Lane 1: CDC14A transfected lysate(66.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CDC14A on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CDC14A
Entrez GeneID
8556GeneBank Accession#
NM_003672Protein Accession#
NP_003663Gene Name
CDC14A
Gene Alias
cdc14, hCDC14
Gene Description
CDC14 cell division cycle 14 homolog A (S. cerevisiae)
Omim ID
603504Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
CDC10 (cell division cycle 10, S. cerevisiae, homolog)|CDC14 homolog A|OTTHUMP00000012653|OTTHUMP00000012654|OTTHUMP00000012655|dual specificity protein phosphatase CDC14A
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Interactome
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Pathway
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Disease
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