MAPKAPK5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MAPKAPK5 transfected lysate.
Western Blot
Lane 1: MAPKAPK5 transfected lysate ( 54.00 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MAPKAPK5 full-length
Host
Human
Theoretical MW (kDa)
54
Interspecies Antigen Sequence
Mouse (97); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MAPKAPK5 antibody (H00008550-D01P) by Western Blots.
SDS-PAGE Gel
MAPKAPK5 transfected lysate.
Western Blot
Lane 1: MAPKAPK5 transfected lysate ( 54.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MAPKAPK5
Entrez GeneID
8550GeneBank Accession#
NM_003668.2Protein Accession#
NP_003659.2Gene Name
MAPKAPK5
Gene Alias
PRAK
Gene Description
mitogen-activated protein kinase-activated protein kinase 5
Omim ID
606723Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
p38-regulated/activated protein kinase
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Interactome
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Pathway
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