PIR 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
PIR transfected lysate.
Western Blot
Lane 1: PIR transfected lysate ( 32.01 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PIR full-length
Host
Human
Theoretical MW (kDa)
32.01
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PIR antibody (H00008544-B01) by Western Blots.
SDS-PAGE Gel
PIR transfected lysate.
Western Blot
Lane 1: PIR transfected lysate ( 32.01 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PIR
Entrez GeneID
8544GeneBank Accession#
NM_003662.2Protein Accession#
-Gene Name
PIR
Gene Alias
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Gene Description
pirin (iron-binding nuclear protein)
Omim ID
603329Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
OTTHUMP00000022961|OTTHUMP00000022962|pirin
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