Western blot analysis of PIR over-expressed 293 cell line, cotransfected with PIR Validated Chimera RNAi ( Cat # H00008544-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIR monoclonal antibody (M03), clone 4D1 (Cat # H00008544-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.[supplied by OMIM