Western blot analysis of PIR over-expressed 293 cell line, cotransfected with PIR Validated Chimera RNAi ( Cat # H00008544-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIR monoclonal antibody (M03), clone 4D1 (Cat # H00008544-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq