DDO 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DDO transfected lysate.
Western Blot
Lane 1: DDO transfected lysate ( 40.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDO full-length
Host
Human
Theoretical MW (kDa)
40.7
Interspecies Antigen Sequence
Mouse (80); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDO antibody (H00008528-B01) by Western Blots.
SDS-PAGE Gel
DDO transfected lysate.
Western Blot
Lane 1: DDO transfected lysate ( 40.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDO
Entrez GeneID
8528GeneBank Accession#
NM_003649.2Protein Accession#
NP_003640.2Gene Name
DDO
Gene Alias
DASOX, DDO-1, DDO-2, FLJ45203
Gene Description
D-aspartate oxidase
Omim ID
124450Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017000|OTTHUMP00000017001|OTTHUMP00000017002|aspartic oxidase
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