PPM1D (Human) Recombinant Protein (P01)
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More Files
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Specifications
Product Description
Human PPM1D full-length ORF (NP_003611.1, 1 a.a. - 605 a.a.) recombinant protein with GST tag at N-terminal.
Sequence
MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPTAEEKPSPRRSLSQPLPPRPSPAALPGGEVSGKGPAVAAREARDPLPDAGASPAPSRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHKPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLEEDPWPRVNSKDHIPALVRSNAFSENFLEVSAEIARENVQGVVIPSKDPEPLEENCAKALTLRIHDSLNNSLPIGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRKTVCVC
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
93.1
Interspecies Antigen Sequence
Mouse (87); Rat (89)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — PPM1D
Entrez GeneID
8493GeneBank Accession#
NM_003620.2Protein Accession#
NP_003611.1Gene Name
PPM1D
Gene Alias
PP2C-DELTA, WIP1
Gene Description
protein phosphatase 1D magnesium-dependent, delta isoform
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq
Other Designations
p53-induced protein phosphatase 1|protein phosphatase 1D|protein phosphatase 2C delta isoform|protein phosphatase Wip1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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p21 WAF1/CIP1 promotes p53 protein degradation by facilitating p53-Wip1 and p53-Mdm2 interaction.
Jihyun Lee, Jongdoo Kim, Eun Mi Kim, Ukjin Kim, A-Ram Kang, Jong Kuk Park, Hong-Duck Um.
Biochemical and Biophysical Research Communications 2021 Mar; 543:23.
Application:PI, WB-Re, Human, HCT-116 cells, Recombinant proteins.
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Wip1 controls the translocation of the chromosomal passenger complex to the central spindle for faithful mitotic exit.
Xianghua Zhang, Ji Eun Park, Eun Ho Kim, Jihee Hong, Ki-Tae Hwang, Young A Kim, Chang-Young Jang.
Cellular and Molecular Life Sciences : CMLS 2021 Mar; 78(6):2821.
Application:Func, Human, HeLa cells.
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p21 WAF1/CIP1 promotes p53 protein degradation by facilitating p53-Wip1 and p53-Mdm2 interaction.
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