SMARCA5 monoclonal antibody (M02), clone 3F4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SMARCA5.
Immunogen
SMARCA5 (NP_003592, 59 a.a. ~ 147 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EEIFDDASPGKQKEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGDYRHRRTE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SMARCA5 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SMARCA5 is approximately 3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SMARCA5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — SMARCA5
Entrez GeneID
8467GeneBank Accession#
NM_003601Protein Accession#
NP_003592Gene Name
SMARCA5
Gene Alias
ISWI, SNF2H, WCRF135, hISWI, hSNF2H
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Omim ID
603375Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq
Other Designations
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a5|sucrose nonfermenting-like 5
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