CUL4B 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CUL4B full-length
Host
Human
Theoretical MW (kDa)
100.54
Interspecies Antigen Sequence
Mouse (97); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CUL4B antibody (H00008450-B01) by Western Blots.
Western Blot
Lane 1: CUL4B transfected lysate ( 104 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CUL4B transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CUL4B
Entrez GeneID
8450GeneBank Accession#
NM_003588Protein Accession#
NP_003579Gene Name
CUL4B
Gene Alias
DKFZp686F1470, KIAA0695, MRXHF2, MRXSC, SFM2
Gene Description
cullin 4B
Gene Ontology
HyperlinkGene Summary
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023946|OTTHUMP00000023947|cullin-4B
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Interactome
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Pathway
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