EEA1 monoclonal antibody (M03), clone 2G2
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More Files
- More Functions
Western Blot (Cell lysate)
EEA1 monoclonal antibody (M03), clone 2G2. Western Blot analysis of EEA1 expression in A-431 ( Cat # L015V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EEA1 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to EEA1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EEA1.
Immunogen
EEA1 (NP_003557, 1312 a.a. ~ 1411 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
EEA1 monoclonal antibody (M03), clone 2G2. Western Blot analysis of EEA1 expression in A-431 ( Cat # L015V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EEA1 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EEA1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — EEA1
Entrez GeneID
8411GeneBank Accession#
NM_003566Protein Accession#
NP_003557Gene Name
EEA1
Gene Alias
MST105, MSTP105, ZFYVE2
Gene Description
early endosome antigen 1
Omim ID
605070Gene Ontology
HyperlinkGene Summary
162kD|early endosome-associated protein
Other Designations
early endosome antigen 1, 162kD|early endosome-associated protein
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Interactome
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Pathway
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Publication Reference
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Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains.
Pastuszka MK, Okamoto CT, Hamm-Alvarez SF, Andrew MacKay J.
Advanced Functional Materials 2014 Sep; 24(34):5340.
Application：IF, Mouse, CHO cells.
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Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains.
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