SPOP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SPOP transfected lysate.
Western Blot
Lane 1: SPOP transfected lysate ( 41.25 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SPOP full-length
Host
Human
Theoretical MW (kDa)
41.25
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SPOP antibody (H00008405-B01) by Western Blots.
SDS-PAGE Gel
SPOP transfected lysate.
Western Blot
Lane 1: SPOP transfected lysate ( 41.25 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SPOP
Entrez GeneID
8405GeneBank Accession#
NM_001007226.1Protein Accession#
NP_001007227.1Gene Name
SPOP
Gene Alias
TEF2
Gene Description
speckle-type POZ protein
Omim ID
602650Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
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Interactome
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Disease
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