NME5 monoclonal antibody (M04), clone 4F5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NME5.
Immunogen
NME5 (NP_003542, 121 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DLRNALHGSNDFAAAEREIRFMFPEVIVEPIPIGQAAKDYLNLHIMPTLLEGLTELCKQKPADPLIWLADWLLKNNPNKPKLCHHPIVEEPY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (86)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.86 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of NME5 transfected lysate using anti-NME5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NME5 MaxPab rabbit polyclonal antibody.ELISA
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Gene Info — NME5
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Interactome
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Publication Reference
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CCDC146 is required for sperm flagellum biogenesis and male fertility in mice.
Yanjie Ma, Bingbing Wu, Yinghong Chen, Shuang Ma, Liying Wang, Tingting Han, Xiaolei Lin, Fulin Yang, Chao Liu, Jianguo Zhao, Wei Li.
Cellular and Molecular Life Sciences : CMLS 2023 Dec; 81(1):1.
Application:WB, Mouse, NIH3T3 cells.
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CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement.
Chao Liu, Qianchun Wang, Lusheng Gu, Xiuge Wang, Yingying Yin, Tao Huang, Sai Xiao, Shuwen Zhang, Fuqiang Wang, Tao Zhou, Guangqiong Xu, Liying Wang, Fucheng Dong, Jing Jiang, Mengcheng Luo, Jinsong Li, Haobo Zhang, Zi-Jiang Chen, Wei Ji, Baohua Ji, Hongbin Liu, Wei Li.
Current Biology : CB 2023 Aug; 33(16):3371.
Application:IF, Mouse, Mouse spermatozoa.
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CCDC146 is required for sperm flagellum biogenesis and male fertility in mice.
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