CDC45L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CDC45L transfected lysate.
Western Blot
Lane 1: CDC45L transfected lysate ( 65.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDC45L full-length
Host
Human
Theoretical MW (kDa)
65.6
Interspecies Antigen Sequence
Mouse (92); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDC45L antibody (H00008318-D01P) by Western Blots.
SDS-PAGE Gel
CDC45L transfected lysate.
Western Blot
Lane 1: CDC45L transfected lysate ( 65.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CDC45L
Entrez GeneID
8318GeneBank Accession#
NM_003504Protein Accession#
NP_003495.1Gene Name
CDC45L
Gene Alias
CDC45, CDC45L2, PORC-PI-1
Gene Description
CDC45 cell division cycle 45-like (S. cerevisiae)
Omim ID
603465Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. [provided by RefSeq
Other Designations
CDC45 (cell division cycle 45, S.cerevisiae, homolog)-like|CDC45-like|CDC45-related protein|cell division cycle 45-like 2|human CDC45
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Interactome
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Pathway
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Disease
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