GDF5 monoclonal antibody (M06), clone 1F4
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More Files
- More Functions
Western Blot (Cell lysate)
GDF5 monoclonal antibody (M06), clone 1F4. Western Blot analysis of GDF5 expression in HL-60.
Western Blot (Transfected lysate)
Western Blot analysis of GDF5 expression in transfected 293T cell line by GDF5 monoclonal antibody (M06), clone 1F4.
Lane 1: GDF5 transfected lysate (Predicted MW: 55.4 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GDF5 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GDF5.
Immunogen
GDF5 (NP_000548.1, 28 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
APDLGQRPQGTRPGLAKAEAKERPPLARNVFRPGGHSYGGGATNANARAKGGTGQTGGLTQPKKDEPKKLPPRPGGPEPKPGHPPQTRQATARTVTPKGQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (76); Rat (76)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GDF5 monoclonal antibody (M06), clone 1F4. Western Blot analysis of GDF5 expression in HL-60.Western Blot (Transfected lysate)
Western Blot analysis of GDF5 expression in transfected 293T cell line by GDF5 monoclonal antibody (M06), clone 1F4.
Lane 1: GDF5 transfected lysate (Predicted MW: 55.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GDF5 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — GDF5
Entrez GeneID
8200GeneBank Accession#
NM_000557.2Protein Accession#
NP_000548.1Gene Name
GDF5
Gene Alias
BMP14, CDMP1, LAP4, SYNS2
Gene Description
growth differentiation factor 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq
Other Designations
OTTHUMP00000030755|OTTHUMP00000061644|cartilage-derived morphogenetic protein-1
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Interactome
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Pathway
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Disease
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