SYMPK 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SYMPK transfected lysate.
Western Blot
Lane 1: SYMPK transfected lysate ( 58.74 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SYMPK full-length
Host
Human
Theoretical MW (kDa)
58.74
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SYMPK antibody (H00008189-B01) by Western Blots.
SDS-PAGE Gel
SYMPK transfected lysate.
Western Blot
Lane 1: SYMPK transfected lysate ( 58.74 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SYMPK
Entrez GeneID
8189GeneBank Accession#
BC030214.1Protein Accession#
AAH30214.1Gene Name
SYMPK
Gene Alias
FLJ27092, SPK, SYM
Gene Description
symplekin
Omim ID
602388Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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