AKAP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AKAP1 transfected lysate.
Western Blot
Lane 1: AKAP1 transfected lysate ( 99.44 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AKAP1 full-length
Host
Human
Theoretical MW (kDa)
99.44
Interspecies Antigen Sequence
Mouse (68); Rat (68)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AKAP1 antibody (H00008165-B01) by Western Blots.
SDS-PAGE Gel
AKAP1 transfected lysate.
Western Blot
Lane 1: AKAP1 transfected lysate ( 99.44 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AKAP1
Entrez GeneID
8165GeneBank Accession#
BC000729.2Protein Accession#
AAH00729.1Gene Name
AKAP1
Gene Alias
AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, MGC1807, PRKA1, SAKAP84
Gene Description
A kinase (PRKA) anchor protein 1
Omim ID
602449Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq
Other Designations
A-kinase anchor protein 1|A-kinase anchor protein, 149kD|dual-specificity A-kinase anchoring protein 1|protein kinase A anchoring protein 1|protein kinase A1|spermatid A-kinase anchor protein 84
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Interactome
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Disease
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