TAF15 (Human) IP-WB Antibody Pair
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of TAF15 transfected lysate using rabbit polyclonal anti-TAF15 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-TAF15.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-TAF15 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-TAF15 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — TAF15
Entrez GeneID
8148Gene Name
TAF15
Gene Alias
Npl3, RBP56, TAF2N, TAFII68, hTAFII68
Gene Description
TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Omim ID
601574Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
RBP56/CSMF fusion|RNA-binding protein 56|TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68 kD|TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56)|TATA box-binding protein-associ
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Interactome
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Disease
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Publication Reference
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Long non-coding RNA ATXN8OS Promotes Ferroptosis and Inhibits the Temozolomide-resistance of Gliomas Through the ADAR/GLS2 Pathway.
Jin Luo, Rui Bai, Yunxiao Liu, Hong Bi, Xiangzhen Shi, Chongxiao Qu.
Brain Research Bulletin 2022 Aug; 186:27.
Application:RIP-A, Human, U251TR cells.
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Long non-coding RNA ATXN8OS Promotes Ferroptosis and Inhibits the Temozolomide-resistance of Gliomas Through the ADAR/GLS2 Pathway.
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