PDHX 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDHX full-length
Host
Human
Theoretical MW (kDa)
55.22
Interspecies Antigen Sequence
Mouse (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDHX antibody (H00008050-B01) by Western Blots.
SDS-PAGE Gel
PDHX transfected lysate.
Western Blot
Lane 1: PDHX transfected lysate ( 55.22 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PDHX
Entrez GeneID
8050GeneBank Accession#
BC010389.1Protein Accession#
AAH10389.1Gene Name
PDHX
Gene Alias
DLDBP, E3BP, OPDX, PDX1, proX
Gene Description
pyruvate dehydrogenase complex, component X
Gene Ontology
HyperlinkGene Summary
The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
Other Designations
E3-binding protein|pyruvate dehydrogenase complex, lipoyl-containing component X
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