CSRP3 monoclonal antibody (M03), clone 6D2
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant CSRP3.
Immunogen
CSRP3 (NP_003467, 95 a.a. ~ 194 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SPKPARSVTTSNPSKFTAKFGESEKCPRCGKSVYAAEKVMGGGKPWHKTCFRCAICGKSLESTNVTDKDGELYCKVCYAKNFGPTGIGFGGLTQQVEKKE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CSRP3 monoclonal antibody (M03), clone 6D2. Western Blot analysis of CSRP3 expression in Hela S3 NE.Western Blot (Transfected lysate)
Western Blot analysis of CSRP3 expression in transfected 293T cell line by CSRP3 monoclonal antibody (M03), clone 6D2.
Lane 1: CSRP3 transfected lysate(20.969 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CSRP3 over-expressed 293 cell line, cotransfected with CSRP3 Validated Chimera RNAi ( Cat # H00008048-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSRP3 monoclonal antibody (M03), clone 6D2 (Cat # H00008048-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to CSRP3 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CSRP3
Entrez GeneID
8048GeneBank Accession#
NM_003476Protein Accession#
NP_003467Gene Name
CSRP3
Gene Alias
CLP, CMD1M, CMH12, CRP3, LMO4, MGC14488, MGC61993, MLP
Gene Description
cysteine and glycine-rich protein 3 (cardiac LIM protein)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
LIM domain only 4|cardiac LIM domain protein|cardiac LIM protein|cysteine and glycine-rich protein 3|cysteine-rich protein 3|muscle LIM protein
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Interactomes
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Diseases
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