ARHGEF5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ARHGEF5 full-length
Host
Human
Theoretical MW (kDa)
60.1
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ARHGEF5 antibody (H00007984-D01P) by Western Blots.
SDS-PAGE Gel
ARHGEF5 transfected lysate.
Western Blot
Lane 1: ARHGEF5 transfected lysate ( 60.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ARHGEF5
Entrez GeneID
7984GeneBank Accession#
Q12774Protein Accession#
Q12774Gene Name
ARHGEF5
Gene Alias
DKFZp686N1969, GEF5, P60, TIM, TIM1
Gene Description
Rho guanine nucleotide exchange factor (GEF) 5
Omim ID
600888Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq
Other Designations
guanine nucleotide regulatory protein TIM|rho guanine nucleotide exchange factor 5|transforming immortalized mammary oncogene
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Interactome
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Disease
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