ARHGEF5 monoclonal antibody (M02), clone 4D10-D11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M02), clone 4D10-D11.
Lane 1: ARHGEF5 transfected lysate (Predicted MW: 60.1 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of ARHGEF5 transfected lysate using anti-ARHGEF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF5 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARHGEF5 is 3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (82.83 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ARHGEF5.
Immunogen
ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (82.83 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M02), clone 4D10-D11.
Lane 1: ARHGEF5 transfected lysate (Predicted MW: 60.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ARHGEF5 transfected lysate using anti-ARHGEF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF5 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARHGEF5 is 3 ng/ml as a capture antibody.ELISA
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Gene Info — ARHGEF5
Entrez GeneID
7984GeneBank Accession#
BC014555Protein Accession#
AAH14555Gene Name
ARHGEF5
Gene Alias
DKFZp686N1969, GEF5, P60, TIM, TIM1
Gene Description
Rho guanine nucleotide exchange factor (GEF) 5
Omim ID
600888Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq
Other Designations
guanine nucleotide regulatory protein TIM|rho guanine nucleotide exchange factor 5|transforming immortalized mammary oncogene
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Interactome
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Disease
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