ARHGEF5 monoclonal antibody (M01), clone 3A12-B5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ARHGEF5.
Immunogen
ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (82.83 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M01), clone 3A12-B5.
Lane 1: ARHGEF5 transfected lysate(60.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARHGEF5 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — ARHGEF5
Entrez GeneID
7984GeneBank Accession#
BC014555Protein Accession#
AAH14555Gene Name
ARHGEF5
Gene Alias
DKFZp686N1969, GEF5, P60, TIM, TIM1
Gene Description
Rho guanine nucleotide exchange factor (GEF) 5
Omim ID
600888Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq
Other Designations
guanine nucleotide regulatory protein TIM|rho guanine nucleotide exchange factor 5|transforming immortalized mammary oncogene
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Interactome
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Disease
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Publication Reference
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Identification of gains on 1q and epidermal growth factor receptor overexpression as independent prognostic markers in intracranial ependymoma.
Mendrzyk F, Korshunov A, Benner A, Toedt G, Pfister S, Radlwimmer B, Lichter P.
Clinical Cancer Research 2006 Apr; 12(7 Pt 1):2070.
Application:IHC, Human, well-characterized Human intracranial ependymomas.
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Identification of gains on 1q and epidermal growth factor receptor overexpression as independent prognostic markers in intracranial ependymoma.
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