HSD17B8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
HSD17B8 transfected lysate.
Western Blot
Lane 1: HSD17B8 transfected lysate ( 28.82 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HSD17B8 full-length
Host
Human
Theoretical MW (kDa)
28.82
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HSD17B8 antibody (H00007923-B02) by Western Blots.
SDS-PAGE Gel
HSD17B8 transfected lysate.
Western Blot
Lane 1: HSD17B8 transfected lysate ( 28.82 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HSD17B8
Entrez GeneID
7923GeneBank Accession#
NM_014234.3Protein Accession#
NP_055049.1Gene Name
HSD17B8
Gene Alias
D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, SDR30C1, dJ1033B10.9
Gene Description
hydroxysteroid (17-beta) dehydrogenase 8
Omim ID
601417Gene Ontology
HyperlinkGene Summary
In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq
Other Designations
17-beta-HSD 8|17-beta-hydroxysteroid dehydrogenase 8|OTTHUMP00000029153|beta-ketoacyl-[acyl-carrier-protein] reductase-like|estradiol 17 beta-dehydrogenase 8|estrogen 17-oxidoreductase|short chain dehydrogenase/reductase family 30C, member 1
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Interactome
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Pathway
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Disease
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