RNF103 monoclonal antibody (M01), clone 3E7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RNF103.
Immunogen
RNF103 (NP_005658, 501 a.a. ~ 599 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
HPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNSPGTASHCDAEACSCANKYCQTSPCERKGRSYGSYN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (83)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.
Lane 1: RNF103 transfected lysate(79.405 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi ( Cat # H00007844-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 (Cat # H00007844-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — RNF103
Entrez GeneID
7844GeneBank Accession#
NM_005667Protein Accession#
NP_005658Gene Name
RNF103
Gene Alias
KF1, MGC102815, MGC41857, ZFP103, hkf-1
Gene Description
ring finger protein 103
Omim ID
602507Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING-H2 finger, a motif known to be involved in protein-protein and protein-DNA interactions. This gene is highly expressed in normal cerebellum, but not in the cerebral cortex. The expression of the rat counterpart in the frontal cortex and hippocampus was shown to be induced by elctroconvulsive treatment (ECT) as well as chronic antidepressant treatment, suggesting that this gene may be a molecular target for ECT and antidepressants. [provided by RefSeq
Other Designations
Zinc finger protein expressed in cerebellum|zinc finger protein 103 homolog
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