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NPHS2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00007827-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-NPHS2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 34.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-NPHS2 antibody (H00007827-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00007827-T01
    NPHS2 transfected lysate.
    Western Blot
    QC Testing of H00007827-T01
    Lane 1: NPHS2 transfected lysate ( 34.4 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 7827
  • Gene Name:
  • NPHS2
  • Gene Alias:
  • PDCN,SRN1
  • Gene Description:
  • nephrosis 2, idiopathic, steroid-resistant (podocin)
  • Gene Summary:
  • This gene encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000033186,podocin
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