NPHS2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NPHS2 full-length
Host
Human
Theoretical MW (kDa)
34.4
Interspecies Antigen Sequence
Mouse (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NPHS2 antibody (H00007827-B01) by Western Blots.
SDS-PAGE Gel
NPHS2 transfected lysate.
Western Blot
Lane 1: NPHS2 transfected lysate ( 34.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NPHS2
Entrez GeneID
7827GeneBank Accession#
BC029141Protein Accession#
AAH29141Gene Name
NPHS2
Gene Alias
PDCN, SRN1
Gene Description
nephrosis 2, idiopathic, steroid-resistant (podocin)
Gene Ontology
HyperlinkGene Summary
This gene encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000033186|podocin
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Interactome
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