NPHS2 (Human) IP-WB Antibody Pair
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (80); Rat (77)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of NPHS2 transfected lysate using rabbit polyclonal anti-NPHS2 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-NPHS2.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-NPHS2 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-NPHS2 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — NPHS2
Entrez GeneID
7827Gene Name
NPHS2
Gene Alias
PDCN, SRN1
Gene Description
nephrosis 2, idiopathic, steroid-resistant (podocin)
Gene Ontology
HyperlinkGene Summary
This gene encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000033186|podocin
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Interactome
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Disease
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